By Edward A. Dennis (Eds.)

This quantity on Phospholipases, the 1st of its sort, provides thoughts for the assay, purification, and characterization of those enzymes, which aren't merely degradative enzymes of lipid metabolism yet also are key enzymes within the sign transduction pathway

Show description

Read Online or Download Phospholipases PDF

Similar molecular biology books

Receptor Molecular Biology

The volumes during this sequence comprise modern ideas major to a specific department of neuroscience. they're a useful relief to the coed in addition to the skilled researcher not just in constructing protocols in neuroscience yet in disciplines the place learn is turning into heavily concerning neuroscience.

Methods in Muscle Biology

Equipment in Muscle Biology is a complete laboratory advisor that info the equipment utilized in the learn of muscle biology. The suggestions incorporated embody telephone, developmental, and molecular biology, in addition asphysiology, neurobiology, and scientific study.

Principles and Technical Aspects of PCR Amplification

Kary Mullis was once provided a Nobel Prize for inventing the PCR method greater than 15 years in the past in 1993. because its "discovery", a number of diversifications and diversifications of the traditional PCR strategy were defined, with lots of those diversifications and adaptations at present getting used in scientific, diagnostic and educational laboratories internationally.

Cells Molecules and Mechanisms

The textbook for mobile and molecular biology classes.

Extra resources for Phospholipases

Example text

W. Vogel, A. Plfickthun, H. J. Muller-Eberhard, and E. A. Dennis, Anal. Biochem. 118, 262 (1981). 73 H. S. Hendrickson, E. K. Hendrickson, and T. J. Rustard, J. Lipid Res. 28, 864 (1987). 74 F. Radvanyi, L. Jordan, F. Russo-Marie, and C. Bon, Anal. Biochem. 177, 103 (1989). 20 PHOSPHOLIPASE ASSAYS, KINETICS, SUBSTRATES [1] substrates and may require either the addition or exclusion of albumin and other lipid-binding proteins which can pull labeled fatty acids out of the vesicles into solution.

2 I. C. P. Smith, Can. J. Biochem. 57, l (1979). 3 A. A. Ribeiro and E. A. Dennis, Biochemistry 14, 3746 (1975). 4 R. A. , and M. F. Roberts, Biochemistry 19, 3100 (1980). P. L. Yeagle, in "Phosphorus NMR in Biology" (C. T. ), p. 95. CRC Press, Boca Raton, Florida, 1978. 6 E. A. Dennis, Arch. Biochem. Biophys. 158, 485 0973). 7 M. Y. E1-Sayed, C. D. DeBose, L. A. Coury, and M. F. Roberts, Biochim. Biophys. Acta 837, 325 (1985). 8 C. A. Kensil and E. A. Dennis, J. Biol. Chem. 254, 5843 (1979). 9 N.

15 p. Patriarca, S. Beckerdite, and P. Eisbach, Biochim. Biophys. Acta 260, 593 (1972). 16R. Franson, S. Beckerdite, P. Wang, M. Waite, and P. Elsbach, Biochim. Biophys. Acta 296, 365 (1973). 26 PHOSPHOLIPASE ASSAYS, KINETICS, SUBSTRATES [2] position (>95%) of the E. coli phospholipids 17 provides an easy means of distinguishing positional preference of deacylating activities tested by analysis of labeled products of hydrolysis. (6) Since many deacylating phospholipases are more readily detected when phosphatidylethanolamine is the substrate than with phosphatidylcholine, the autoclaved E.

Download PDF sample

Rated 4.08 of 5 – based on 14 votes