By Edward A. Dennis (Eds.)
This quantity on Phospholipases, the 1st of its sort, provides thoughts for the assay, purification, and characterization of those enzymes, which aren't merely degradative enzymes of lipid metabolism yet also are key enzymes within the sign transduction pathway
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Extra resources for Phospholipases
W. Vogel, A. Plfickthun, H. J. Muller-Eberhard, and E. A. Dennis, Anal. Biochem. 118, 262 (1981). 73 H. S. Hendrickson, E. K. Hendrickson, and T. J. Rustard, J. Lipid Res. 28, 864 (1987). 74 F. Radvanyi, L. Jordan, F. Russo-Marie, and C. Bon, Anal. Biochem. 177, 103 (1989). 20 PHOSPHOLIPASE ASSAYS, KINETICS, SUBSTRATES  substrates and may require either the addition or exclusion of albumin and other lipid-binding proteins which can pull labeled fatty acids out of the vesicles into solution.
2 I. C. P. Smith, Can. J. Biochem. 57, l (1979). 3 A. A. Ribeiro and E. A. Dennis, Biochemistry 14, 3746 (1975). 4 R. A. , and M. F. Roberts, Biochemistry 19, 3100 (1980). P. L. Yeagle, in "Phosphorus NMR in Biology" (C. T. ), p. 95. CRC Press, Boca Raton, Florida, 1978. 6 E. A. Dennis, Arch. Biochem. Biophys. 158, 485 0973). 7 M. Y. E1-Sayed, C. D. DeBose, L. A. Coury, and M. F. Roberts, Biochim. Biophys. Acta 837, 325 (1985). 8 C. A. Kensil and E. A. Dennis, J. Biol. Chem. 254, 5843 (1979). 9 N.
15 p. Patriarca, S. Beckerdite, and P. Eisbach, Biochim. Biophys. Acta 260, 593 (1972). 16R. Franson, S. Beckerdite, P. Wang, M. Waite, and P. Elsbach, Biochim. Biophys. Acta 296, 365 (1973). 26 PHOSPHOLIPASE ASSAYS, KINETICS, SUBSTRATES  position (>95%) of the E. coli phospholipids 17 provides an easy means of distinguishing positional preference of deacylating activities tested by analysis of labeled products of hydrolysis. (6) Since many deacylating phospholipases are more readily detected when phosphatidylethanolamine is the substrate than with phosphatidylcholine, the autoclaved E.