By Lelio Orci M.D., Alain Perrelet M.D. (auth.)

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Extra info for Freeze-Etch Histology: A Comparison between Thin Sections and Freeze-Etch Replicas

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What remains of them is only their bases which are slightly raised above the fracture face. The fractured bases of microvilli must be clearly distinguished from the endothelial pores described in Plates 5 to 9. The fact that the broken microvillar bases are raised above the A-face of the membrarie and that they enclose the cytoplasmic matrix might be used as significant criteria for distinction. A large area of the follicular lumen (*) appears in the lower right-hand part of the picture. It contains several profiles of intact microvilli.

53, 758-776 (1972). , ORCI, L. : A freeze-etch study of the tight junctions of the rat kidney tubules. Lab. Invest. 30, 286-291 (1974). 40 Plate 19 41 Differentiations of the Cell Surface in Freeze-Etching Plate 20 Endocrine Cells from Human Pancreas (Isolated Islet of Langerhans) In this highly magnified picture, the characteristic appearance of the tight junctional elements is emphasized. The fracture plane has exposed large areas of the A-face of a cell membrane. This face carries linear ridges (TJ) which are interrupted only by focal discontinuities.

Such altered areas are transient and will disappear owing to redistribution of the particles in the plane of the membrane (see reference in Plate 23: SINGER and NICOLSON, 1972). Magnification x 78,000. From L. ORCI, Diabetologia 10, 1-25 (1974). : Insulin release by emiocytosis: demonstration with freeze-etching technique. Science 179,82-84 (1973). : Exocytosis in adrenal medulla demonstrated by freeze-etching. Science 179, 79-82 (1973). * However, it is not possible to exclude that the disappearance of particles is an event preceding or accompanying the fusion of the granule's membrane with the plasma membrane.

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