By Hans J. Vogel

Calcium-binding proteins play a major position in a number of important organic tactics, starting from blood clotting and sign transduction in cells, to attaching proteins to membranes and serving as an essential resource of calcium. In Calcium-Binding Protocols-Volume 1: experiences and Case stories and quantity 2: tools and Techniques-Hans Vogel and a panel of top researchers evaluation the protein chemistry and behaviour of this crucial protein type, and supply a complete number of confirmed experimental innovations for his or her research either in vitro and in vivo. this primary quantity discusses the position of calcium in intracellular secondary messenger activation mechanisms, together with certain elements of calcium chemistry and its usage in dairy proteins and blood clotting. distinct case experiences supply a wealth of precious information regarding protein purification and characterization concepts, X-ray crystallography, and particular calcium-binding proteins and their modes of motion. the second one better half quantity, tools and strategies, specializes in state-of-the-art experimental equipment for learning resolution constitution, balance, dynamics, calcium-binding houses, and organic job of calcium-binding proteins in general.
finished and hugely sensible, the 2 volumes of Calcium-Binding Protocols offer experimental and medical biologists with a number of complex experimental equipment that may be utilized effectively to the examine of either latest and newly came upon participants of this significantly vital classification of proteins.

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Extra resources for Calcium-Binding Protein Protocols: Volume 1: Reviews and Case Studies

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They are likely to be localized differences both along a membrane and across it of its negatively charged headgroups. Together, with localized membrane curvature, these differences will lead to corresponding variations in associated calciumion concentration. A recent atomic force microscopy study shows the way Ca2+ induces coalescence of lipids into domains (see ref. 9). However, because the calcium ions have pumps and channels forcing or allowing Ca2+ ion flow, the 34 Williams Fig. 6. The calcium currents around the terminal of a T-tubule of the sarcoplasmic reticulum.

And Vogel H. J. (1998) Calcium-calmodulin-induced dimerization of the carboxyl-terminal domain from petunia glutamate decarboxylase. A novel calmodulin-peptide interaction motif. J. Biol. Chem. 273, 30,328–30,335. 62. , Gronenborn, A. , Klee, C. , and Bax, A. (1992) Solution structure of a calmodulin-target peptide complex by multidimensional NMR. Science 256, 632–638. 63. Meador, W. , Means, A. , and Quiocho, F. A. 4 Å structure of a calmodulin-peptide complex. Science 257, 1251–1255. 64. Meador, W.

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