By Daniel Herschlag

This MIE quantity presents laboratory options that goal to foretell the constitution of a protein that could have great implications starting from drug layout, to mobile pathways and their dynamics, to viral access into cells.
Expert researchers introduce the main complex applied sciences and strategies in protein constitution and folding
Includes concepts on tiling assays.

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Additional info for Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part B

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Can be detected and appropriate troubleshooting conducted.  If binding of the RNA to the column appears to be weak, the above procedure can be adjusted. Transcription and addition of HMM protein are done as described above, but then the solution is passed over the NiNTA resin at 4  C and allowed to sit for 5–10 min; to ensure complete binding of the RNA/protein complex to the column, the flow-through can be passed through a second time. In the cold room, the column is washed four times with 4 CV of cold RNA column buffer.

Ligand requirements for glmS ribozyme self-cleavage. Chem. Biol. 12, 1221–1226. Native Purification of RNAs 25 McKenna, S. , Puglisi, E. , Lindhout, D. , Aitken, C. , Marshall, R. , and Puglisi, J. D. (2007). Purification and characterization of transcribed RNAs using gel filtration chromatography. Nat. Protoc. 2, 3270–3277. Milligan, J. , Groebe, D. , Witherell, G. , and Uhlenbeck, O. C. (1987). Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res.

Application of solution equilibrium analysis to in vitro RNA transcription. Biotechnol. Prog. 13, 747–756. Kieft, J. , and Batey, R. T. (2004). A general method for rapid and nondenaturing purification of RNAs. RNA 10, 988–995. , McKenna, S. , and Puglisi, J. D. (2007). Rapid purification of RNAs using fast performance liquid chromatography (FPLC). RNA 13, 289–294. , and Peabody, D. S. (1994). Mutations that increase the affinity of a translational repressor for RNA. Nucleic Acids Res. 22, 3748–3752.

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