By Thomas N. Wight and Robert P. Mecham (Eds.)

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A n d Poole, A. R. (1984). Trans. Orthop. Res. Soc. 9, 3 1 3 . H a r d i n g h a m , Τ. , and Muir, Η. (1973). Biochem. J. 135, 9 0 5 - 9 0 8 . , and H a s c a l l , V. C. (1974). J. Biol. Chem. 2 5 4 , 9 2 7 - 9 3 4 . Wight, Τ. , and H a s c a l l , V. C. (1983). J. Cell Biol. 9 6 , 1 6 7 - 1 7 6 . S t e v e n s , R. , E w i n s , R. J. , Revell, P. , and Muir, Η. (1979). Biochem. J. 179, 561-572. 26 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. BRUCE CATERSON ET AL. H e i n e g a r d , D .

Revell, P. , and Muir, Η. (1979). Biochem. J. 179, 561-572. 26 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. BRUCE CATERSON ET AL. H e i n e g a r d , D . , a n d V o g e l , K. (1985). Biochem. J. 2 3 0 , 1 8 1 - 1 9 4 . S t e v e n s , J. , H a n d l e y , C. , H a s c a l l , V. , a n d Caterson, Β. (1984). J. Cell Biochem. 2 6 , 2 4 7 - 2 5 9 . N e a m e , P. , P e r i n , J - P . , Christner, J. E . , a n d B a k e r , J. R. (1985). J. Biol. Chem. 2 6 0 , 1 2 4 0 2 - 1 2 4 0 4 . H a r d i n g h a m , Τ.

The later technique is often called hybrid-selected translation. In an alternative host-vector system recently developed by Young and Davis (1983), double-stranded cDNA is inserted within t h e ß-galactosidase structural gene, lacZ, of the bacteriophage expression vec­ tor, Xgtll. When expression of the lacZ gene is induced, the eukaryotic DNA will be transcribed and translated, producing a hybrid molecule which will contain the amino acid sequence of both proteins. The hybrid protein produced by a bacterial colony can be identified by reaction with antibodies to the protein of interest.

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