By Alton Meister

Ribonuclease P: An Enzyme with a Catalytic RNA Subunit (S. Altman).

law of Escherichia coli Glutamine Synthetase (S. Rhee, et al.).

Glucose 6-Phosphatase: options of Membrane-Function courting (K. Sukalski & R. Nordlie).

Chiral Phosphorothioates: Stereochemical research of Enzymatic Substitution at Phosphorus (P. Frey).

Serotonin and Peptide Immunoneuromodulators: contemporary Discoveries and New principles (D. Silverman & M. Karnovsky).

The Phosphyglycerate Mutases (L. Fothergill-Gilmore & H. Watson).

Mechanism and law of the Glutamine-Dependent Carbamyl Phosphate Synthetase of Escherichia coli (A. Meister).

writer Index.

topic Index.

Cummulative Index, Vols.

1-62.Content:

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Extra resources for Advances in Enzymology and Related Areas of Molecular Biology, Volume 62

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B) Both cations induce the same ultraviolet spectral change when added to the relaxed enzyme. (c) Binding of Mg(I1) or Mn(I1) to the nl site causes a rapid (complete in less than 3 sec) release of about one equivalent of H C followed by a slow first-order release of a second H + (57). 022 sec-I at 37°C. It should be pointed out that binding of either Mg(I1) or Mn(I1) to the n2 site also causes a rapid release of one equivalent of H + , but no slow H + release step was observed (57). 2) at 37°C for the nl-metalM and -5 x lop5 M for Mn(I1) and cation complex is -5 x Mg(II), respectively.

Coli enzyme is very similar to that of the Salmonella enzyme (25), somewhat different from the Anabaena enzyme (26), and distinctly different from the mammalian enzyme (27). Electron-microscopic study (28) has shown that the 12 subunits in each molecule are arranged in a double-decked hexagon, with a REGULATION OF ESCHERICHIA cOLIGLUTAMINE SYNTHETASE 41 sixfold dihedral symmetry. The outer diameter of the hexagon is 140 x 45 x 53 A (see Fig. 1). The x-ray crystallographic study of the E. 5%i-resolutionelectron density has been reported for the Salmonella enzyme (29), which crystallized isomorphously with the E.

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