By Arthur Clarkson
A textual content - ebook of Histology: Descriptive and functional offers info pertinent to the overall equipment of histology. This publication systematically describes the constitution of the tissue or organ.
Organized into 17 chapters, this publication starts off with an summary of the constitution of the physique as obvious below the microscope. this article then discusses the final tools of staining reagents, clearing, mounting, and injection of blood vessels. different chapters clarify the various levels in karyokinesis, together with community or resting degree, convolution, wreath, monaster, and dyaster. This publication discusses in addition the microscopical exam of amphibian and mammalian blood and examines the results of reagents on human blood. the ultimate bankruptcy examines the human spinal wire stained with aniline.
This ebook is a invaluable source for college kids.
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Additional resources for A Text-Book of Histology. Descriptive and Practical. For the Use of Students
Without, in both cases dissolving the paraffin with turpentine, on the slide, and mount ing in balsam. Then practise getting rid of the paraffin, and transferring to water before mounting, both with stained and GENERAL METHODS. 35 unstained specimens, the latter requiring to be stained in the same way as sections cut in gum. Next, starting with a piece of tissue already preserved in spirit, put it through the various stages of staining, with borax-carmine, decolorising with acid alcohol, dehydrating with absolute alco hol, infiltrating with turpentine and paraffin, and finally cut it in the Cambridge rocker or Minot's microtome.
C. saturated solution of picric acid. The stain is diffuse and acts rapidly. Sections after staining require immersion in acid alcohol. —The strong ammoniacal solution of carmine de scribed above may be kept in stock, and diluted according to the strength required. The solution should be filtered before using, in order to remove any precipitate which may have fallen from over-evaporation of the ammonia. , before they are cut into sections, especially for those which are to be cut in paraffin. It is one of the diffuse stains, however, colouring all the elements of the tissue too uniformly: In order, therefore, to use it as a nuclear stain, place the piece of tissue in acid alcohol, which will dissolve out the stain to a great extent, from all but the nuclei of the cells, which remain deeply coloured.
Be employed for clarifying the sections. The paraffin should be removed with turpentine, and the specimen mounted directly in balsam. Another method of fixation is also frequently employed. The sections are floated in ribands of suitable length upon the surface of warm water, when they become completely flattened out. The temperature should not be sufficiently high, however, to melt the soft paraffin which unites their edges, as the ribbons then fall to pieces. They are then floated into the required position on the slide, and the superfluous water removed with a clean rag or blotting paper.